L-.alpha.-glycerophosphate oxidase gene, recombinant DNA, and method for producing modified L-.alpha.-glycerophosphate oxidase gene
US6303357A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 29, 2000 |
| Grant date | Oct 16, 2001 |
| Priority date | — |
| Expiry date | Mar 29, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/0006
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides an L-.alpha.-glycerophosphate oxidase (GPO) having excellent properties such as stability, heat resistance and reactivity. A recombinant GPO obtained by replacing an amino acid of a specified position of an amino acid sequence deduced from the Enterococcus faecium No. 7044 GPO gene or DNA coding for the GPO with other amino acid has excellent thermal stability and reactivity. That is, the invention provides modified forms of the GPO having the amino acid sequence of Sequence No. 1 in the Sequence Listing, in which the 130-position leucine counting from the N-terminus of the GPO is replaced by other amino acid and/or the 225-position serine counting from the N-terminus of the GPO is replaced by other amino acid and/or the 298-position threonine counting from the N-terminus of the GPO is replaced by other amino acid and/or the 420-position aspartic acid counting from the N-terminus of the GPO is replaced by other amino acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.