Methods of targeted mutagenesis using triple-helix forming oligonucleotides
US6303376A · kind A · utility
10Cited by
1References
12Claims
0Family size
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Key dates
| Filing date | Oct 4, 1999 |
| Grant date | Oct 16, 2001 |
| Priority date | — |
| Expiry date | Oct 4, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/3511
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A high affinity, triplex-forming oligonucleotide and methods for use thereof wherein an oligonucleotide is used to form a triple-stranded nucleic acid molecule with a specific DNA segment of a target DNA molecule. Upon formation of the triplex, the binding of the oligonucleotide stimulates mutagenesis within or adjacent to the target sequence using cellular DNA synthesis or repair mechanisms. The mutation activates, inactivates or alters the activity and function of the target molecule.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.