Temperature sensitive plasmid for coryneform bacteria
US6303383A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 8, 2000 |
| Grant date | Oct 16, 2001 |
| Priority date | — |
| Expiry date | Mar 8, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/77
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A coryneform bacterium in which a DNA fragment is incorporated into its chromosome is prepared by (a) obtaining a recombinant plasmid through ligation of a DNA fragment having a sequence homologous to a gene present on a chromosome of a coryneform bacterium to a plasmid that has a wild-type replication control region segment of a particular nucleotide sequence including a mutation and is autonomously replicable in a coryneform bacterium cell at a culture temperature lower than 31.degree. C. but not autonomously replicable in the cell at a temperature of 31.degree. C. or higher, (b) introducing the recombinant plasmid into the coryneform bacterium cell, (c) culturing the bacterium at a temperature of 31.degree. C. or higher, (d) causing homologous recombination between the DNA fragment and the gene present on the chromosome of the coryneform bacterium and having a sequence homologous to the DNA fragment, and (e) selecting a coryneform bacterium in which the DNA fragment is incorporated into its chromosome. According to the present invention, there is provided a method for efficiently modifying genetic traits of a host in a short period of time by obtaining a temperature sensitive pl…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.