Detection and treatment of duplex polynucleotide damage
US6306601A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 22, 1999 |
| Grant date | Oct 23, 2001 |
| Priority date | — |
| Expiry date | Nov 22, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07F15/008
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Duplex polynucleotides containing damage or errors are detected with hindered intercalating compounds which are capable of intercalating only in the presence of such damage or error. Conditions characterized by the presence of polynucleotide errors or damage are treated with such compounds that are capable of catalyzing polynucleotide cleavage with light. Suitable compound include compounds of the formula: EQU Rh(R.sub.1)(R.sub.2)(R.sub.3).sup.3+ where R.sub.1 and R.sub.2 are each indepenently aryl, heteroaryl, substituted aryl or substituted heteroaryl of 1 to 5 rings, and R.sub.3 is a group of the formula ##STR1## where x and z are each independently an interger from 1 to 4 and y is an interger from 1 to 2, and R.sub.4, R.sub.5, and R.sub.6 are independently H--, halo, HO--, H.sub.2 N--, CN--, O.sub.2 N--, HS--, O.sub.3 S--, O.sub.3 SO--, --COOH, --CONH.sub.2, R, RO--, RNH--, R.sub.a R.sub.b N--, RO.sub.3 SO--, .sub.RO.sub.3 SO--, --COOR, --CONHR, or --CONHR.sub.a R.sub.b where R, R.sub.a and R.sub.b are each independently lower alkyl, cycloalkyl, lower alkenyl, lower alkynyl, or phenyl, or two R.sub.4, R.sub.5, or R.sub.6 together form a fused aryl ring.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.