Modulators of leaderless protein export and methods for identifying and using the same
US6306613A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 1, 1999 |
| Grant date | Oct 23, 2001 |
| Priority date | — |
| Expiry date | Dec 1, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/68
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Methods of modulating the export of a leaderless protein from a cell by contacting the cell with a compound that alters the binding of the leaderless protein and a transport molecule are provided. Transport molecules include gastrin binding protein/alpha subunit of mitochondrial fatty acid .beta.-oxidation multienzyme complex (p70, GenBank Accession Nos. U04627/D16480), phosphotyrosine-independent ligand of the SH2 domain of p56lck (p62, GenBank Accession No. U46751), mitochondrial fatty acid .beta.-oxidation trifunctional protein .beta. subunit (TP-.beta.) (p48, GenBank Accession No. D16481), actin related protein 3 (Arp3) (p48, GenBank Accession No. U29610), K-glypican (GenBank Accession No. X83577), tubulin (p50, GenBank Accession No. AF081484) and related polypeptides that are functionally equivalent in their role as leaderless protein trafficking components. Leaderless proteins include, for example, FGF-1, FGF-2, IL-1.alpha., IL-1.beta., CNTF, MIF, and HIV tat. These methods are useful in treatment of various conditions, including tumors and diabetes as well is in identifying small molecules for export modulation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.