Clarification of protein precipitate suspensions using anionic polymeric flocculants
US6307013A · kind A · utility
23Cited by
18References
35Claims
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Key dates
| Filing date | Jul 16, 1999 |
| Grant date | Oct 23, 2001 |
| Priority date | — |
| Expiry date | Jul 16, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/61
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Protein suspensions comprising soluble and insoluble components are purified via flocculation with anionic polymers, such as polyacrylamides, potato starch, or modified cellulose. The procedure improves the efficiency of solid/liquid separations and can minimize or eliminate the requirement for centrifugation and/or filtration in large scale biotechnological processes. The method is particularly well suited for the purification and clarification of protein suspensions containing soluble somatotropin monomer using polyacrylamide and polysaccharide flocculants.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.