Seeding parenchymal cells into compression resistant porous scaffold after vascularizing in vivo
US6309635A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Nov 28, 1994 |
| Grant date | Oct 30, 2001 |
| Priority date | — |
| Expiry date | Nov 28, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/40
- WIPO fieldMedical technology
- WIPO sectorInstruments
Abstract
A method is provided whereby cells having a desired function are seeded on and into biocompatible, biodegradable or non-degradable porous polymer scaffolding matrix, previously implanted in a patient and infiltrated with blood vessels and connective tissue, to produce a functional organ equivalent. The resulting organoid is a chimera formed of parenchymal elements of the donated tissue and vascular and matrix elements of the host. The matrix should be compression resistant and a non-toxic, porous template for vascular ingrowth. The pore size, usually between approximately 100 and 300 microns, should allow vascular and connective tissue ingrowth throughout approximately 10 to 90% of the matrix, and the injection of cells such as hepatocytes without damage to the cells or patient. The introduced cells attach to the connective tissue and are fed by the blood vessels. Immediately prior to matrix implantation portacaval shunts can be created to provide trophic stimulatory factors to the implanted matrix to enhance replication and function.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.