Process for isolation of hepatoprotective agent silymarin from the seeds of the plant Silybum marianum
US6309678A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 31, 2000 |
| Grant date | Oct 30, 2001 |
| Priority date | — |
| Expiry date | Mar 31, 2020 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61P1/16
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention relates to a novel process for the isolation of a hepatoprotective agent Silymarin from the seeds of the plant Silybum marianum comprising (i) Precooling the seeds to (-) 20.degree. C. for 24 hours in a deep freezer/cold room. (ii) Powdering the cooled seeds in a hammer mill, fitted with about 40 mesh discharge screen. (iii) Defatting the seeds by extracting with hexane in a soxhlet type extractor to remove the total quantity of fatty oil without using a scrain. (iv) Extracting the defatted seeds with acetonitrile at 20-30.degree. C. to extract silymarin fraction. (v) Concentration of the sensitive silymarin fraction under vacuum in a agitated wiped thin film evaporator (vi) stirring the silymarin such dry powder with cold dichloromethane at 5.degree. C. followed by filtration and drying with a slow purge of nitrogen gas. (vii) Further purification of silymarin by suspending in 5 times its weight of acetonitrile and precipitating by 8-12 times its weight of water at 20.degree. C. (viii) Filtering the precipitated silymarin in a closed vacuum filter and having 1-2 .mu.m screen washing the cake three times with distilled water (ix) Drying of Silymarin cake in vacuum ove…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.