Multiplex amplification and separation of nucleic acid sequences on a bioelectronic microchip using asymmetric structures
US6309833A · kind A · utility
39Cited by
26References
47Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 12, 1999 |
| Grant date | Oct 30, 2001 |
| Priority date | — |
| Expiry date | Apr 12, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2565/607
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for amplifying nucleic acids is provided wherein detection of amplified species is enhanced by the use of asymmetric amplification. Such amplification is made asymmetric by using divergent ratios of amplification primers or by using non-extending and/or non-cleavable amplification primers. Detection of the amplicons is improved because maintenance of single stranded species of amplicons during amplification facilitates their direct capture by immobilized probes without having to include denaturing steps.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.