Screening methods for compounds that inhibit or stimulate helicase enzyme activity
US6309839A · kind A · utility
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12Claims
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Key dates
| Filing date | Mar 30, 2000 |
| Grant date | Oct 30, 2001 |
| Priority date | — |
| Expiry date | Mar 30, 2020 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/20
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The gp4l and Dda helicases were found to significantly enhance the dissociation rate of streptavidin from biotin-labeled oligonucleotides in an ATP dependent reaction, demonstrating that these enzymes are capable of imparting a significant force on a molecule blocking their path. The present invention describes an assay for studying enzymatic activity of a helicase using the rate of dissociation of streptavidin from biotinylated oligonucleotides.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.