High fidelity detection of nucleic acid differences by ligase detection reaction
US6312892A · kind A · utility
64Cited by
9References
88Claims
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Key dates
| Filing date | Jul 10, 1997 |
| Grant date | Nov 6, 2001 |
| Priority date | — |
| Expiry date | Jul 10, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6862
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Ligase detection reaction is utilized to distinguish minority template in the presence of an excess of normal template with a thermostable ligase. This process can be carried out with a mutant ligase, thermostable ligase, or a modified oligonucleotide probe. This procedure is particularly useful for the detection of cancer-associated mutations. It has the advantage of providing a quantitative measure of the amount or ratio of minority template.
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