Methods and compositions for determining the sequence of nucleic acid molecules
US6312893A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 22, 1997 |
| Grant date | Nov 6, 2001 |
| Priority date | — |
| Expiry date | Jul 22, 2017 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/24
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Methods and compounds, including compositions therefrom, are provided for determining the sequence of nucleic acid molecules. The methods permit the determination of multiple nucleic acid sequences simultaneously. The compounds are used as tags to generate tagged nucleic acid fragments which are complementary to a selected target nucleic acid molecule. Each tag is correlative with a particular nucleotide and, in a preferred embodiment, is detectable by mass spectrometry. Following separation of the tagged fragments by sequential length, the tags are cleaved from the tagged fragments. In a preferred embodiment, the tags are detected by mass spectrometry and the sequence of the nucleic acid molecule is determined therefrom. The individual steps of the methods can be used in automated format, e.g., by the incorporation into systems.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.