Methods and compositions to facilitate D-loop formation by oligonucleotides
US6312925A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 7, 1998 |
| Grant date | Nov 6, 2001 |
| Priority date | — |
| Expiry date | May 7, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/3513
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and compositions for efficient targeting and modification of target sequences in duplex DNA are provided, utilizing oligonucleotides or oligonucleotide compositions containing two domains. The first domain comprises an entity capable of recognizing a double-stranded DNA sequence. This can be a protein, peptide, antibiotic, minor groove binding agent or a nucleotide sequence capable of triplex formation The second domain, which is covalently joined to the first, is capable of recognizing a single-stranded DNA sequence. This second domain will most often be complementary, in the Watson-Crick sense, to a target sequence in the double-stranded nucleic acid. The second domain can optionally carry one or more modifying groups, capable of causing a mutation, a pre-mutagenic lesion, or some other type of heritable change in the target sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.