Solid phase synthesis

US6313284A · kind A · utility

Inventors

• Marek Kwiatkowski · Uppsala, SE
• Ulf Landegren · Uppsala, SE
• Mats Nilsson · Uppsala, SE

Key dates

• Filing date: Nov 12, 1999
• Grant date: Nov 6, 2001
• Expiry date: Nov 12, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6834
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

A method of preparing an immobilized oligonucleotide having a free 3'-end comprises the steps of: i) preparing an oligonucleotide attached in a first position to a solid support via its 3'-end and having a free 5'-end; ii) binding said oligonucleotide in a second position remote from the 3'-end to the solid support; and iii) selectively releasing the 3'-end of the oligonucleotide from the solid support to obtain the oligonucleotide attached to the support in said second position in a reversed orientation with a free 3'-end.