Asporogenic B anthracis expression system
US6316006A · kind A · utility
9Cited by
0References
4Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 23, 1994 |
| Grant date | Nov 13, 2001 |
| Priority date | — |
| Expiry date | Nov 23, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/07
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to a bacterial expression system for production of protective antigen (PA) against bacillus anthracis. Recombinant asporogenic B. anthracits that are derived from .DELTA.Sterne-1(pPA102) and show inability to bind the dye when grown on Congo Red Agar can be screened and asporogenic strains isolated using methods of the invention. organisms of the invention lacking spore-forming function may be killed by heat shock at temperatures as low as 60.degree. C. for 60 minutes. Hence, contamination of the environment with viable spore-forming organisms is easily avoided and decontamination is easily accomplished.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.