Mutant cells of Pichia
US6316245A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 26, 1999 |
| Grant date | Nov 13, 2001 |
| Priority date | — |
| Expiry date | Aug 26, 2019 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/938
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a fermentation process to have a high productivity with a novel mutant of Pichia sp., more specifically, for preparing erythritol under optimal fermentation conditions for maximum erythritol production by optimizing the environmental conditions of culture such as pH, temperature and by controlling osmotic pressure. A two-stage fermentation was performed to control osmotic pressure. Osmotic pressure was adjusted to a low level during growth phase and to a relatively high level during production phase by adding continuously glucose and NaCl or KCl. Therefore, erythritol production could be increased.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.