"Preparation and use of gene banks of human antibodies (""human-antibody libraries"")"

US6319690A · kind A · utility

Assignee

• Dade Behring Marburg GmbH · DE

Inventors

• Melvyn Little · Büdelsdorf, DE
• Frank Breitling · Heidelberg, DE
• Thomas Seehaus · Heppenheim, DE
• Stefan Dubel · Dossenheim, DE
• Iris Klewinghaus · Mannheim, DE

Key dates

• Filing date: May 6, 1993
• Grant date: Nov 20, 2001
• Expiry date: May 6, 2013

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/02
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention relates to the preparation and use of gene banks of human antibodies (Ab). Starting from a mixture of human B-lymphocytes, their mRNA is translated into the cDNA using oligo-dT primers. Subsequently, an amplification of the Ab-specific cDNA by means of polymerase chain reaction (PCR) takes place using suitable oligonucleotide primer sequences. Expression of this amplified Ab-specific cDNA in a bacterial expression vector, e.g. the vector pFMT described below, in E. coli thus makes available a human-antibody library with a comprehensive repertoire for screening selected antigens in vitro.