(S)-hydroxynitrile lyases with improved substrate acceptance and their use

US6319697A · kind A · utility

Assignee

• DSM Fine Chemicals Austria GmbH · AU

Inventors

• Franz Effenberger · Stuttgart, DE
• Harald Wajant · Echterdingen, DE
• Peter Lauble · Berlin, DE
• Siegfried Forster · Alsdorf, DE
• Holger Buhler · Ludwigsburg, DE
• Helmut Schwab · Graz, AT
• Christoph Kratky · Graz, AT
• Ulrike Wagner · Graz, AT
• Ernst Steiner · Richterswil, CH

Key dates

• Filing date: Jul 1, 1999
• Grant date: Nov 20, 2001
• Expiry date: Jul 1, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/88
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

(S)-Hydroxynitrile lyases which have an improved substrate acceptance and which are derived from the Hevea brasiliensis and Manihot esculenta (S)-hydroxynitrile lyases, wherein one or more bulky amino acid residues within the hydrophobic channel leading to the active center have been replaced with less bulky amino acid residues.