Oligonucleotide mediated nucleic acid recombination

US6319714A · kind A · utility

Assignee

• Maxygen, Inc. · US

Inventors

• Andreas Crameri · Los Altos Hills, US
• Willem P. C. Stemmer · Los Gatos, US
• Jeremy Minshull · Los Altos, US
• Steven H. Bass · Hillsborough, US
• Mark Welch · Fremont, US
• Jon E. Ness · Sunnyvale, US
• Claes Gustafsson · Belmont, US
• Phillip A. Patten · Portola Valley, US

Key dates

• Filing date: Jul 27, 2000
• Grant date: Nov 20, 2001
• Expiry date: Jul 27, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2740/16122
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.