Sterol-regulated Site-1 protease and assays of modulators thereof

US6322962A · kind A · utility

Assignee

• The Board of Regents of the University of Texas System · US

Inventors

• Michael S. Brown · Dallas, US
• Dong Cheng · Furlong, US
• Peter J. Espenshade · Dallas, US
• Joseph L. Goldstein · Dallas, US
• Robert B. Rawson · Lewisville, US
• Juro Sakai · Rifu, JP

Key dates

• Filing date: Jul 23, 1999
• Grant date: Nov 27, 2001
• Expiry date: Jul 23, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides assays for the identification of modulators of Site-1 protease. Further provided by the invention are expression constructs and the transgenic cells useful for the development of such assays for Site-1 specific protease. The cells allow the implementation of in vitro assays for potential modulators of Site-specific proteases. Still further provided by the invention are in vitro assays employing Site-1 protease which has been isolated from cells.