Methods for modifying the production of a polypeptide
US6323002A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 25, 1999 |
| Grant date | Nov 27, 2001 |
| Priority date | — |
| Expiry date | Jun 25, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/01004
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to methods for modifying the production of a polypeptide, comprising: (a) introducing a nucleic acid construct into a cell, wherein the cell comprises a DNA sequence encoding a polypeptide, under conditions in which the nucleic acid construct integrates into the genome of the cell at a locus not within the DNA sequence encoding the polypeptide to produce a mutant cell, wherein the integration of the nucleic acid construct modifies the production of the polypeptide by the mutant cell relative to the cell when the mutant cell and the cell are cultured under the same conditions; and (b) identifying the mutant cell with the modified production of the polypeptide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.