Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6323030A · kind A · utility
183Cited by
89References
26Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Jan 29, 1999 |
| Grant date | Nov 27, 2001 |
| Priority date | — |
| Expiry date | Jan 29, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2317/622
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.