Patent · US Expired

Method of determining multiple mRNAs in dying cells

US6326146A · kind A · utility

1Cited by

0References

2Claims

0Family size

Inventors

Dianne M. O'Dell · Drexel Hill, US
Ramesh Raghupathi · Devon, US
Tracy Kahl McIntosh · Wallingford, US
Peter B. Crino · Overland Park, US
James Eberwine · Philadelphia, US

Key dates

Filing date	Mar 23, 1999
Grant date	Dec 4, 2001
Priority date	—
Expiry date	Mar 23, 2019

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q2600/158
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A method for determining expression levels of multiple mRNAs in single, dying cells from a selected tissue is provided. The method utilizes terminal deoxynucleotidyl-transferase mediated biotin-dUTP nick end labeling to identify dying cells and measures multiple mRNA expression levels in single, isolated dying cells or portions thereof by amplified antisense RNA techniques.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.