Vector for gene transfer into plant allowing optional deletion of marker gene
US6326192A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 9, 1998 |
| Grant date | Dec 4, 2001 |
| Priority date | — |
| Expiry date | Nov 9, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8242
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a vector for introducing a desired gene into a plant, wherein a selectable marker gene introduced into a plant cell along with a desired gene is optionally removable from the DNA such as chromosome or the like where it exists and functions, then disappeared the function thereof after its expression, and the expression of the selectable marker gene and the disappearance of the function thereof are detectable by morphological change of the tissue derived from the plant cell into which the selectable marker gene is introduced. Also, the present invention constitutes a vector using a morphological abnormality induction gene as a selectable marker gene, while putting a removable DNA element under control of an inducible promoter, wherein the morphological abnormality induction gene is positioned such that it behaves integrally with the removable DNA element, and wherein a desired gene is positioned such that it does not behave integrally with the removable DNA element.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.