Nitrate reductase-transfected HeLa cells for cancer and microwave bioeffects research
US6326196A · kind A · utility
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1Claims
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Key dates
| Filing date | Jan 22, 2001 |
| Grant date | Dec 4, 2001 |
| Priority date | — |
| Expiry date | Jan 22, 2021 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/502
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The cell line HeLa is transformed with the chromosomal insertion of the plasmid pSV.sub.2 neoNR10.sub.1, ATCC No. 69617. The transformed cells, HeLaNR1, produce diazoluminomelanin (DALM) intra cellularly when provided with nitrate, luminol and 3-amino-L-tyrosine.cndot.HC1 (3AT). The modified cells can be used to study mechanisms for radiofrequency and light radiation interactions with carcinoma of the cervix. The effects of drugs, hormones, and cytokines that affect the expression of nitric oxide synthase and its activity can also be studied to understand the effects of these materials on cervix cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.