Solid phase native chemical ligation of unprotected or n-terminal cysteine protected peptides in aqueous solution
US6326468A · kind A · utility
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Key dates
| Filing date | Jun 12, 1998 |
| Grant date | Dec 4, 2001 |
| Priority date | — |
| Expiry date | Jun 12, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K1/047
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides methods, apparatus and kits for synthesizing assembled peptides and proteins on a solid phase with sequential ligation of three or more unprotected peptide segments using chemoselective and mild ligation chemistries in aqueous solution. Also provided are methods of monitoring solid phase sequential ligation reactions using MALDI or electrospray ionization mass spectrometry of reaction products.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.