Patent · US Expired

Use of dimly fluorescing nucleic acid dyes in the identification of nucleated cells

US6329158A · kind A · utility

33Cited by

6References

13Claims

0Family size

Assignee

Becton, Dickinson and Company · US

Inventors

Robert A. Hoffman · Mansfield, US
Thomas Frey · Friolzheim, DE

Key dates

Filing date	Sep 15, 1995
Grant date	Dec 11, 2001
Priority date	—
Expiry date	Sep 15, 2015

Classification

Technology area (CPC G)Physics
CPC primaryG01N33/56972
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

This invention presents improved methodology for identification of nucleated cells in flow cytometric analysis when immunofluorescent dyes are also used. Briefly, in the method nucleic acids are stained with a fluorescent dye which can then be used to identify the nucleated cells by measurement of fluorescence on a flow cytometer. The improvement presented by this invention is the use of a saturating (or near saturating) amount of a nucleic acid dye, or mixture of dyes, which gives low fluorescence at excitation conditions, so as not to greatly interfere with the signals of the immunofluorescent dyes.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.