ABC transporter gene cluster in pseudomonas fluorescens for enhanced lipase secretion
US6329172A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 26, 2000 |
| Grant date | Dec 11, 2001 |
| Priority date | — |
| Expiry date | Jan 26, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/21
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides ABC transporter gene of P. fluorescens encoding a protein which enhances the secretion of foreign proteins extracellularly, a recombinant expression vector containing the transporter gene, a microorganism transformed therewith and a process for preparing foreign proteins which comprises the steps of incubating the transformant, inducing expression of the proteins and leading the proteins to be secreted extracellularly. In accordance with the present invention, foreign proteins can be secreted extracellularly in a simple and efficient manner by employing a secretion system associated with the ABC transporter gene.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.