High lysine fertile transgenic corn plants
US6329574A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 24, 1998 |
| Grant date | Dec 11, 2001 |
| Priority date | — |
| Expiry date | Jul 24, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A40/146
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stablefs transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.