Arrays for identifying agents which mimic or inhibit the activity of interferons
US6331396A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 23, 1999 |
| Grant date | Dec 18, 2001 |
| Priority date | — |
| Expiry date | Sep 23, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/158
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and model systems for identifying and characterizing new therapeutic agents, particularly proteins, which mimic or inhibit the activity of all interferons, Type I interferons, IFN-.alpha., IFN-.beta., or IFN-.gamma.. The method comprises administering an interferon selected from the group consisting of IFN-.alpha., IFN .beta., IFN-.tau., IFN-.omega., IFN-.gamma., and combinations thereof to cultured cells, administering the candidate agent to a duplicate culture of cells; and measuring the effect of the candidate agent and the interferon on the transcription or translation of one or, preferably, a plurality of the interferon stimulated genes or the interferon repressed genes (hereinafter referred to as "ISG's" and "IRGs", respectively). The model system is an array with gene probes that hybridize with from about 100 to about 5000 ISG and IRG transcripts.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.