Cytochrome P450 monooxygenase and NADPH cytochrome P450 oxidoreductase genes and proteins related to the omega hydroxylase complex of Candida tropicalis and methods relating thereto
US6331420A · kind A · utility
Inventors
Key dates
| Filing date | Apr 30, 1999 |
| Grant date | Dec 18, 2001 |
| Priority date | — |
| Expiry date | Apr 30, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P7/44
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the .omega.-hydroxylase complex of C. tropicalis 20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.