Method for directional stable transformation of eukaryotic cells
US6331661A · kind A · utility
45Cited by
1References
47Claims
0Family size
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Key dates
| Filing date | Nov 17, 1998 |
| Grant date | Dec 18, 2001 |
| Priority date | — |
| Expiry date | Nov 17, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/53
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is drawn to compositions and methods for introducing nucleotide sequences at preferred genomic target sites in a eukaryotic genome. The compositions comprise transfer cassettes which are flanked by nonhomologous recombination sites. The method involves transforming eukaryotic cells containing target sites utilizing non-integrating transformation methods. The method results in efficient integration of nucleotides into predetermined genetic locations and eliminates random DNA integration.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.