Process for obtaining microorganisms containing peptide amidase, microorganisms obtained therewith, peptide amidases contained in them and use thereof

US6333176A · kind A · utility

Assignee

• Degussa AG · DE

Inventors

• Ursula Stelkes-Ritter · Niederzier, DE
• Maria-Regina Kula · Jülich, DE
• Klaudia Wyzgol · Düsseldorf, DE
• Andreas Bommarius · Frankfurt, DE
• Michael Schwarm · Alzenau, DE
• Karlheinz Drauz · Alzenau, DE

Key dates

• Filing date: Aug 25, 1999
• Grant date: Dec 25, 2001
• Expiry date: Aug 25, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12R2001/64
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A plant peptide amidase from the flavedo of oranges is known; this can be obtained only in small quantities and is seasonal. The proposed new enzymes are microbial peptide amidases which can be obtained from micro-organisms recovered from soil samples in a "double screening" and grown. These microbial peptide amidases are particularly useful for (a) the production of peptides and N-terminal-protected amino acids, (b) racemate splitting of N-protected amino acid amides, (c) obtaining non-proteinogenous D-amino acids, and (d) obtaining new N.sub..alpha. -protected D-amino acid amides.