Methods and compositions for multiplex amplification of nucleic acids
US6333179A · kind A · utility
20Cited by
4References
13Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 18, 1998 |
| Grant date | Dec 25, 2001 |
| Priority date | — |
| Expiry date | Jun 18, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method is described for predetermining ratios of primer pairs present in a single reaction vessel so as to achieve approximately equimolar yield of products. The ratios are determined as a function of the length of the amplicon and the length of other amplicons being simultaneously tested. The primers may desirably be for p53 gene sequences.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.