Linked linear amplification of nucleic acids
US6335184B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 11, 1999 |
| Grant date | Jan 1, 2002 |
| Priority date | — |
| Expiry date | Jan 11, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2549/119
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The extensive synthesis (“amplification”) of a nucleic acid sequence of interest is attained through a linked series of multi-cycle primer extension reactions (LLA). The primers used in each of the primer extension reactions of the process contain non-replicable and/or cleavable elements that halt nucleic acid synthesis and thereby prevent the synthesized molecules from serving as templates in subsequent cycles. Synthesized molecules accumulate during primer extension in a mathematically linear fashion, thereby rendering the process relatively insensitive to contaminating nucleic acids. Multiple primer sets are employed, and simultaneously included in the reaction mixture, thereby ensuring the accumulation of a large number of copies of the nucleic acid sequence of interest. The invention also provides for the detection of an amplified nucleic acid sequence of interest, as well as reagent kits for carrying out the reaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.