Method for cloning and producing the BsmI restriction endonuclease in E. coli
US6335190B1 · kind B1 · utility
19Cited by
2References
6Claims
0Family size
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Key dates
| Filing date | Oct 20, 2000 |
| Grant date | Jan 1, 2002 |
| Priority date | — |
| Expiry date | Oct 20, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/1007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to recombinant DNA which encodes the BsmI restriction endonuclease as well as BsmI methyltransferases, expression of BsmI restriction endonuclease in E. coil cells containing the recombinant DNA by using a low copy number T7 expression vector pACYC-T7ter, and purification of BsmI restriction endonuclease by heat treatment and chromatography through heparin Sepharose column.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.