Gene for enzyme having both alkaline pullulanase and alkaline &agr;-amylase activities
US6338959B1 · kind B1 · utility
1Cited by
2References
8Claims
0Family size
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Key dates
| Filing date | Feb 28, 2000 |
| Grant date | Jan 15, 2002 |
| Priority date | — |
| Expiry date | Feb 28, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/01041
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a DNA fragment encoding alkaline pullulanase exhibiting alkaline &agr;-amylase activity, alkaline &agr;-amylase possessing both an amino acid sequence described in SEQ ID NO:3 and a DNA fragment encoding the amylase, alkaline pullulanase possessing both an amino acid sequence described in SEQ ID NO:4 and a DNA fragment encoding the pullulanase, recombinant DNAs containing these DNA fragments, and transformed microorganisms harboring the recombinant DNAs. The technique of the present invention enables mass production of alkaline pullulanase exhibiting alkaline &agr;-amylase activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.