Process and medium for mammalian cell culture under low dissolved carbon dioxide concentration

US6338964B1 · kind B1 · utility

Assignee

• BAYER HEALTHCARE LLC · DE

Inventors

• Ricaredo Matanguihan · Walnut Creek, US
• Eva Sajan · Oakland, US
• Konstantin Konstantinov · Newton, US
• Michael Zachariou · Walnut Creek, US
• Charles Olson · Moraga, US

Key dates

• Filing date: May 7, 1999
• Grant date: Jan 15, 2002
• Expiry date: May 7, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2500/60
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A cell culture medium which is low in dissolved carbon dioxide is disclosed. The medium contains less than about 1 g/L added sodium bicarbonate and includes an organic buffer and a metal complexing agent. The medium is preferably essentially free of added sodium bicarbonate. Methods of use of the medium in culturing mammalian cells, particularly cells engineered to produce recombinant factor VIII, are also disclosed.