Production of desiccation-tolerant gymnosperm embryos
US6340594B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 5, 1997 |
| Grant date | Jan 22, 2002 |
| Priority date | — |
| Expiry date | Dec 5, 2017 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01H4/006
- WIPO fieldFood chemistry
- WIPO sectorChemistry
Abstract
Desiccated mature gymnosperm somatic embryos, preferably coniferous somatic embryos having a moisture content of less than about 55%, are prepared by water stressing immature embryos in a medium comprising a metabolizable carbon source and a suitable growth regulator influencing embryo development, for a period of time and under conditions sufficient to yield mature viable somatic embryos having a moisture content in the specified range. The water stressing should may be environmental, as by controlled declining relative humidity. Or a suitable osmotic or non-osmotic water stress agent can be used. The use of a non-permeating osmotic water stress agent such as polyethylene glycol of molecular weight at least about 1,000 is preferred, in which case the water stress should simulate a non-osmotic water stress. An example of a suitable preferred growth regulator is abscisic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.