Identification of genes
US6342215B1 · kind B1 · utility
Assignees
Inventors
Key dates
| Filing date | Dec 1, 1998 |
| Grant date | Jan 29, 2002 |
| Priority date | — |
| Expiry date | Dec 1, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A50/30
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method for identifying a microorganism having a reduced adaptation to a particular environment comprising the steps of:(1) providing a plurality of microorganisms each of which is independently mutated by the insertional inactivation of a gene with a nucleic acid comprising a unique marker sequence so that each mutant contains a different marker sequence, or clones of the said microorganism;(2) providing individually a stored sample of each mutant produced by step (1) and providing individually stored nucleic acid comprising the unique marker sequence from each individual mutant;(3) introducing a plurality of mutants produced by step (1) into the said particular environment and allowing those microorganisms which are able to do so to grow in the said environment;(4) retrieving microorganisms from the said environment or a selected part thereof and isolating the nucleic acid from the retrieved microorganisms;(5) comparing any marker sequences in the nucleic acid isolated in step (4) to the unique marker sequence of each individual mutant stored as in step (2); and(6) selecting an individual mutant which does not contain any of the marker sequences as isolated in step (4).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.