Microfluidic method for nucleic acid purification and processing

US6344326B1 · kind B1 · utility

Assignee

• Aclara Biosciences, Inc. · US

Inventors

• Robert J. Nelson · Sanford, US
• Herbert H. Hooper · Belmont, US
• Alan K. Hauser · Palo Alto, US
• Sharat Singh · San Jose, US
• Stephen J. Williams · Scottsdale, US
• Alexander Sassi · Berkeley, US

Key dates

• Filing date: Feb 10, 2000
• Grant date: Feb 5, 2002
• Expiry date: Feb 10, 2020

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01L2400/0421
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Integrated microfluidic devices comprising at least an enrichment channel (10) and a main electrophoretic flowpath (12) are provided. In the subject integrated devices, the enrichment channel and the main electrophoretic flowpath are positioned so that waste fluid flows away from said main electrophoretic flowpath through a discharge outlet (6). The subject devices find use in a variety of electrophoretic applications, including clinical assays, high throughput screening for genomics and pharmaceutical applications, point-or-care in vitro diagnostics, molecular genetic analysis and nucleic acid diagnostics, cell separations, and bioresearch generally.