Real-time monitoring of PCR using LOCI
US6346384B1 · kind B1 · utility
42Cited by
12References
20Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Mar 27, 2000 |
| Grant date | Feb 12, 2002 |
| Priority date | — |
| Expiry date | Mar 27, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6816
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The current invention relates to the use of luminescent oxygen channeling immunoassay (“LOCI”) technology to monitor amplification reactions, especially polymerase chain reactions (“PCR”). More specifically, the current invention involves the use of LOCI to measure the kinetics of a PCR reaction in an all-in-one assay format in order to quantitatively and qualitatively detect a target polynucleotide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.