ATM kinase modulation for screening and therapies
US6348311B1 · kind B1 · utility
Assignees
Inventors
Key dates
| Filing date | Sep 21, 1999 |
| Grant date | Feb 19, 2002 |
| Priority date | — |
| Expiry date | Sep 21, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/9121
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to identification of the consensus sequence phosphorylated by ATM kinase. This, in turn, permitted identification of ATM kinase target proteins, and development of a convenient assay system for ATM kinase phosphorylation using fusion polypeptides as substrates. The assay system is adaptable to screening for ATM modulators, particularly inhibitors. In a specific embodiment, the substrate recognition sequence and mutagenized variants of this sequence were incorporated in a GST fusion protein and assayed for phosphorylation by ATM kinase. This assay system is useful in screening for ATM inhibitors. ATM function assays were validated using an ATM-kinase dead dominant-negative mutant.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.