Polysome display in the absence of functional ssrA-RNA
US6348315B1 · kind B1 · utility
16Cited by
0References
44Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 22, 1999 |
| Grant date | Feb 19, 2002 |
| Priority date | — |
| Expiry date | Oct 22, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K16/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to methods for identifying nucleic acid molecules encoding (poly)peptides that interact with target molecules. The method of the present invention is particularly characterized by an in vitro translation step under conditions that allow formation of polysomes in the presence of antisense oligonucleotides complementary to the tag-coding sequence of ssrA-RNA. The present invention further relates to kits that are useful for carrying out the method of the invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.