Process for purification of PCR test samples
US6348336B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 1, 1997 |
| Grant date | Feb 19, 2002 |
| Priority date | — |
| Expiry date | Jul 1, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6806
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process is provided for preparing samples of blood, blood plasma, blood serum, or plasma proteins, including blood factor products, for PCR testing which minimizes contaminants which may interfere with the analysis. The process includes centrifugation of the initial sample to form a sample pellet, removing at least a portion of the supernatant from the pellet, and washing the pellet with an aqueous buffer. The buffer and washed pellet are then centrifuged, and a portion of the remaining supernatant is removed along with any contaminants contained therein. The clean, substantially contaminant-free pellet is then processed for PCR analysis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.