Recombinant DNA and methods for producing thermostable enzymes
US6350591B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 16, 1999 |
| Grant date | Feb 26, 2002 |
| Priority date | — |
| Expiry date | Feb 16, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/902
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
We have developed a new gene transfer system for extreme thermophiles of the genus Thermus, including Thermus flavus., using a chromosomal gene, and a thermostable derivative of the kanamycin-resistance gene (kantr2). A plasmid mediated gene-replacement process is used to insert it into the chromosome resulting in the production of Leu−Kmr transformants. This system not only allows stable, single-copy gene insertion into the chromosome of an extreme thermophile, but can be used in the thermo-genetic process described here to generate thermo-stabilized enzymes and proteins for industrial processes. This host-vector environment makes it possible to generate further thermo-stabilizing mutations in the kan gene beyond those levels previously reported.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.