Patent · US Expired

Method for the purification of human platelet heparanase

US6352852B1 · kind B1 · utility

1Cited by

5References

6Claims

0Family size

Assignee

The Australian National University · AU

Inventors

Craig Geoffrey Freeman · Weston Creek, AU
Christopher Richard Parish · Campbell, AU

Key dates

Filing date	Jan 24, 2000
Grant date	Mar 5, 2002
Priority date	—
Expiry date	Jan 24, 2020

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY10S435/815
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A method for the purification of human platelet heparanase using chromatographic techniques is described. The method comprises detergent-aided solubilization of a heparanase-containing fraction followed by purification of the heparanase therefrom by concanavalin A, Zn2+-chelating Sepharose, a Blue A or Reactive Red matrix, octyl agarose and gel filtration chromatography. Heparanase so purified has a molecular mass (Mr) of about 50 kDa and degrades both heparin and heparan sulfates.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.