Nucleic acid ligand interaction assays
US6355428B1 · kind B1 · utility
5Cited by
6References
38Claims
0Family size
Assignee
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Key dates
| Filing date | Sep 10, 1999 |
| Grant date | Mar 12, 2002 |
| Priority date | — |
| Expiry date | Sep 10, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6823
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for determining the relative binding affinities of various ligands to various nucleic acid sequences, particularly in double stranded form, are described. In a direct binding assay, the effect of adding increasing amounts of a ligand on a signal generated by two “indicator” oligonucleotides is observed. Also described is a competitive binding assay, in which a competitor oligonucleotide is added to an indicator duplex having a ligand bound thereto. The assays allow the rapid and convenient determination of nucleic acid binding specificities and base pair determinants of specificity of particular ligands.
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