Methods for detecting and enumerating Campylobacter jejuni in environmental samples and for identifying antibiotic-resistant strains
US6355435B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 6, 2000 |
| Grant date | Mar 12, 2002 |
| Priority date | — |
| Expiry date | Sep 6, 2020 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/80
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The present invention provides a process for detecting and enumerating Campylobacter jejuni in an environmental sample. The present invention further provides a process which can distinguish antibiotic resistant strains of Campylobacter jejuni from wild-type strains, in particular, antibiotic-resistant strains resistant to high levels of ciprofloxacin. Both processes use PCR primers which flank a target sequence unique to Campylobacter jejuni in combination with one or more dual-labeled oligonucleotide probes complementary to the target sequence wherein the dual-labeled probes enable detection of PCR amplification by fluorescence detection means.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.