High speed, automated, continuous flow, multi-dimensional molecular selection and analysis
US6358692B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 12, 1999 |
| Grant date | Mar 19, 2002 |
| Priority date | — |
| Expiry date | Mar 12, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/8831
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention provides novel methods for screening a sample to select a ligand to a target of interest and for obtaining information about the ligand and its binding characteristics. Specifically, the claimed multi-dimensional methods involve combining a solution of heterogeneous ligands with the target of interest to screen the ligands on the basis of one or more binding characteristics. Ligands having the first binding characteristic bind to the target of interest thereby to form a target/ligand complex. The complex then optionally is separated from the unbound components using any of a variety of separation techniques, e.g., size exclusion. At least one of the complex or unbound components then is introduced to a second “dimension”. The second dimension is capable of separating components based upon a second binding characteristic. One then elutes the ligand having the desired binding characteristics.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.